1.
Virus Res
; 227: 49-56, 2017 01 02.
Artigo
em Inglês
| MEDLINE
| ID: mdl-27686478
RESUMO
A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10µM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40µM.